Large-scale discovery of induced point mutations with high-throughput TILLING.

@article{Till2003LargescaleDO,
  title={Large-scale discovery of induced point mutations with high-throughput TILLING.},
  author={Bradley J Till and Steven H. Reynolds and Elizabeth A. Greene and Christine A. Codomo and Linda C. Enns and Jessica E. Johnson and Chris Burtner and Anthony R. Odden and Kim Roy Young and Nicholas E. Taylor and Jorja G. Henikoff and Luca Comai and Steven Henikoff},
  journal={Genome research},
  year={2003},
  volume={13 3},
  pages={524-30}
}
TILLING (Targeting Induced Local Lesions in Genomes) is a general reverse-genetic strategy that provides an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and low-cost discovery of induced point mutations in populations of chemically mutagenized individuals. As chemical mutagenesis is widely applicable and mutation detection for TILLING is dependent only on sufficient yield of PCR products, TILLING can be applied to most organisms. We… CONTINUE READING
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