Large-scale characterization of intact N-glycopeptides using an automated glycoproteomic method.

@article{Cheng2014LargescaleCO,
  title={Large-scale characterization of intact N-glycopeptides using an automated glycoproteomic method.},
  author={Kai Cheng and Rui Chen and Deeptee Seebun and Mingliang Ye and Daniel Figeys and Hanfa Zou},
  journal={Journal of proteomics},
  year={2014},
  volume={110},
  pages={
          145-54
        }
}
UNLABELLED The detailed characterization of site-specific glycosylation requires the identification of glycan composition and specific attachment sites on proteins, which need the identification of intact glycopeptides by mass spectrometry. In this study, we present an analytical and computational strategy for the high throughput characterization of intact N-glycopeptides derived from complex proteome samples. N-glycopeptides were identified using the spectra acquired for intact glycopeptides… CONTINUE READING
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