Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression

@article{Okubo1992LargeSC,
  title={Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression},
  author={Kousaku Okubo and N Hori and Ryo Matoba and T. Niiyama and A Fukushima and Yuko Kojima and Kenichi Matsubara},
  journal={Nature Genetics},
  year={1992},
  volume={2},
  pages={173-179}
}
Large scale sequencing of cDNAs provides a complementary approach to structural analysis of the human genome by generating expressed sequence tags (ESTs). We have initiated the large–scale sequencing of a 3′–directed cDNA library from the human liver cell line HepG2, that is a non–biased representation of the mRNA population. 982 random cDNA clones were sequenced yielding more than 270 kilobases. A significant portion of the identified genes encoded secretable proteins and components for… 

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