Large-scale analysis of the yeast proteome by multidimensional protein identification technology

  title={Large-scale analysis of the yeast proteome by multidimensional protein identification technology},
  author={M. Washburn and D. Wolters and J. Yates},
  journal={Nature Biotechnology},
  • M. Washburn, D. Wolters, J. Yates
  • Published 2001
  • Biology, Medicine
  • Nature Biotechnology
  • We describe a largely unbiased method for rapid and large-scale proteome analysis by multidimensional liquid chromatography, tandem mass spectrometry, and database searching by the SEQUEST algorithm, named multidimensional protein identification technology (MudPIT). MudPIT was applied to the proteome of the Saccharomyces cerevisiae strain BJ5460 grown to mid-log phase and yielded the largest proteome analysis to date. A total of 1,484 proteins were detected and identified. Categorization of… CONTINUE READING
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