Large-scale analysis of the yeast proteome by multidimensional protein identification technology

@article{Washburn2001LargescaleAO,
  title={Large-scale analysis of the yeast proteome by multidimensional protein identification technology},
  author={M. Washburn and D. Wolters and J. Yates},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={242-247}
}
  • M. Washburn, D. Wolters, J. Yates
  • Published 2001
  • Biology, Medicine
  • Nature Biotechnology
  • We describe a largely unbiased method for rapid and large-scale proteome analysis by multidimensional liquid chromatography, tandem mass spectrometry, and database searching by the SEQUEST algorithm, named multidimensional protein identification technology (MudPIT). MudPIT was applied to the proteome of the Saccharomyces cerevisiae strain BJ5460 grown to mid-log phase and yielded the largest proteome analysis to date. A total of 1,484 proteins were detected and identified. Categorization of… CONTINUE READING
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    References

    SHOWING 1-10 OF 68 REFERENCES
    Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels.
    • 1,262
    • PDF
    Proteomics to study genes and genomes
    • 2,105
    • PDF
    Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.
    • 1,293
    • PDF
    Two‐dimensional gel protein database of Saccharomyces cerevisiae (update 1999)
    • 116
    A Sampling of the Yeast Proteome
    • 588
    • PDF
    Direct analysis of protein complexes using mass spectrometry
    • 2,089
    • PDF