Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi

@article{Maeda2001LargescaleAO,
  title={Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi},
  author={Ikuma Maeda and Yuji Kohara and Masayuki Yamamoto and Asako Sugimoto},
  journal={Current Biology},
  year={2001},
  volume={11},
  pages={171-176}
}
Genome-wide analysis of gene function is essential for the post-genome era, and development of efficient and economical technology suitable for it has been in demand. Here we report a large-scale inactivation of the expressed genes in the nematode Caenorhabditis elegans. For this purpose, we have established a high-throughput "RNAi-by-soaking" methodology by modifying the conventional RNAi method [1, 2]. A set of tag-sequenced, nonredundant cDNAs corresponding to approximately 10,000 genes [3… Expand

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