Large electrostatic differences in the binding thermodynamics of a cationic peptide to oligomeric and polymeric DNA.

@article{Zhang1996LargeED,
  title={Large electrostatic differences in the binding thermodynamics of a cationic peptide to oligomeric and polymeric DNA.},
  author={Wenli Zhang and Jeffrey P. Bond and Charles F. Anderson and Timothy M. Lohman and M. Thomas Record},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 6},
  pages={2511-6}
}
Results presented here demonstrate that the thermodynamics of oligocation binding to polymeric and oligomeric DNA are not equivalent because of long-range electrostatic effects. At physiological cation concentrations (0.1-0.3 M) the binding of an oligolysine octacation KWK6-NH2 (+8 charge) to single-stranded poly(dT) is much stronger per site and significantly more salt concentration dependent than the binding of the same ligand to an oligonucleotide, dT(pdT)10 (-10 charge). These large… CONTINUE READING