Large aqueous channels in membrane vesicles derived from the rough endoplasmic reticulum of canine pancreas or the plasma membrane of Escherichia coli.

@article{Simon1989LargeAC,
  title={Large aqueous channels in membrane vesicles derived from the rough endoplasmic reticulum of canine pancreas or the plasma membrane of Escherichia coli.},
  author={Sanford M Simon and Gunter Blobel and Joshua Zimmerberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 16},
  pages={6176-80}
}
Voltage clamp conditions were used to study the membrane permeability properties of rough microsomes (RM) derived from the rough endoplasmic reticulum of canine pancreas and inverted vesicles (InV) derived from the plasma membrane of Escherichia coli. Membrane vesicles of RM or InV were fused to a planar lipid bilayer that was formed in a hole of a partition separating two chambers. Fusion of a single RM vesicle yielded a single-step conductance increase. Some preparations yielded unitary… CONTINUE READING

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