Large Scale Isolation and Purification of Eyespot Granules from Euglena gracilis var. bacillaris.

@article{Bartlett1972LargeSI,
  title={Large Scale Isolation and Purification of Eyespot Granules from Euglena gracilis var. bacillaris.},
  author={C J Bartlett and Patricia L. Walne and O. J. Schwarz and D. H. Brown},
  journal={Plant physiology},
  year={1972},
  volume={49 6},
  pages={881-5}
}
Large volumes of eyespot granules were isolated from homogenates of Euglena gracilis Klebs var. bacillaris Pringsheim by flotation centrifugation in a Beckman Ti-15 zonal rotor, and were further purified by centrifugation in a swinging bucket rotor. Examination with the electron microscope showed the eyespot granules to be free from other cellular material. Freezing had no apparent effect on the structure or on the absorption properties of the eyespots. Absorption spectra of pure fractions of… CONTINUE READING