Landscape of genomic alterations in cervical carcinomas.

Abstract

Cervical cancer is responsible for 10-15% of cancer-related deaths in women worldwide. The aetiological role of infection with high-risk human papilloma viruses (HPVs) in cervical carcinomas is well established. Previous studies have also implicated somatic mutations in PIK3CA, PTEN, TP53, STK11 and KRAS as well as several copy-number alterations in the pathogenesis of cervical carcinomas. Here we report whole-exome sequencing analysis of 115 cervical carcinoma-normal paired samples, transcriptome sequencing of 79 cases and whole-genome sequencing of 14 tumour-normal pairs. Previously unknown somatic mutations in 79 primary squamous cell carcinomas include recurrent E322K substitutions in the MAPK1 gene (8%), inactivating mutations in the HLA-B gene (9%), and mutations in EP300 (16%), FBXW7 (15%), NFE2L2 (4%), TP53 (5%) and ERBB2 (6%). We also observe somatic ELF3 (13%) and CBFB (8%) mutations in 24 adenocarcinomas. Squamous cell carcinomas have higher frequencies of somatic nucleotide substitutions occurring at cytosines preceded by thymines (Tp*C sites) than adenocarcinomas. Gene expression levels at HPV integration sites were statistically significantly higher in tumours with HPV integration compared with expression of the same genes in tumours without viral integration at the same site. These data demonstrate several recurrent genomic alterations in cervical carcinomas that suggest new strategies to combat this disease.

DOI: 10.1038/nature12881

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@article{Ojesina2014LandscapeOG, title={Landscape of genomic alterations in cervical carcinomas.}, author={Akinyemi I Ojesina and Lee Lichtenstein and Samuel S . Freeman and Chandra Sekhar Pedamallu and Iv{\'a}n Imaz-Rosshandler and Trevor J. Pugh and Andrew D Cherniack and Lauren Ambrogio and Kristian Cibulskis and Bj\orn Inge Bertelsen and Sandra Lorena Romero-C{\'o}rdoba and Victor Trevino and Karla I Vazquez-Santillan and Alberto Salido Guadarrama and Alexi Anne Wright and Mara W Rosenberg and Fujiko F Duke and Bethany Kaplan and Rui Wang and Elizabeth Nickerson and Heather M. Walline and Michael Lawrence and Chip Stewart and Scott L. Carter and Aaron McKenna and Iram P Rodriguez-Sanchez and Magali Espinosa-Castilla and Kathrine Woie and Line Bjorge and Elisabeth Wik and Mari Kylles\o Halle and Erling A. Hoivik and Camilla Krakstad and Nayeli Belem Gabi{\~n}o and Gabriela Sof{\'i}a G{\'o}mez-Mac{\'i}as and Lezmes D Valdez-Chapa and Mar{\'i}a de Lourdes Garza-Rodr{\'i}guez and German Maytorena and Jorge Vazquez and Carlos Rodea and Adrian Cravioto and Maria L. Cort{\'e}s and H. -G. Greulich and Christopher Paul Crum and Donna S Neuberg and Alfredo Hidalgo-Miranda and Claudia Rangel Escare{\~n}o and Lars Andreas Akslen and Thomas E. Carey and Olav Karsten Vintermyr and Stacey Bolk Gabriel and Hugo Alberto Barrera-Salda{\~n}a and Jorge Melendez-Zajgla and Gad Getz and Helga Birgitte Salvesen and Matthew Meyerson}, journal={Nature}, year={2014}, volume={506 7488}, pages={371-5} }