Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy

  title={Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy},
  author={Rakesh Chander Yashroy},
  journal={Journal of Biosciences},
  • R. C. Yashroy
  • Published 1 June 1990
  • Chemistry
  • Journal of Biosciences
Aqueous dispersions of lipids isolated from spinach chloroplast membranes were studied by electron microscopy after negative staining with phosphotungstic acid. Influence of low temperature (5°C for 24 h) was also investigated. It was observed that when contacted with water, these lipids, as such, formed multilamellar structures. Upon sonication, these multilamellar structures gave rise to a clear suspension of unilamellar vesicles varying in size (diameter) between 250 and 750 Å. When samples… 

Magnetic resonance studies of dynamic organisation of lipids in chloroplast membranes

Spinach chloroplast membranes and aqueous dispersions of their extracted lipids have been studied by spin label (stearic acid) electron spin resonance and carbon-13 nuclear magnetic resonance

13 C-NMR studies of membrane lipid-protein interactions upon protein heat denaturation Summary

Line-broadening of 13C-NMR resonances arising from the 1st (carbonyl), 7th, 9th and 12th carbon atom of fatty-acyl chains with reference to the carbonyl (C-1) group shows increased immobilization of lipid fatty-ACYl chains at these locations, obviously caused by changes in interactions between membrane lipids and proteins upon heat denaturation of membrane proteins.

A Review on Liposomes

This paper summarizes exclusively scalable techniques and focuses on strengths, respectively, limitations in respect to industrial applicability and regulatory requirements concerning liposomal drug formulations based on FDA and EMEA documents.


Liposome’s, sphere-shaped vesicles consisting of on e or more phospholipids bilayers. Today, they are a very useful reproduction, reagent, and tool in various scientif ic disciplines, including

Introductory Chapter: Liposomes - Advances and Perspectives - My Point of View

  • Á. Catala
  • Biology
    Liposomes - Advances and Perspectives
  • 2019
This book offers expert and updated reviews of the field of liposomes and focuses on recent developments in liposome.

Phospholipidomics of human blood microparticles.

The phospholipidome composition of MPs and platelets, collected from the same donors, was found to be qualitatively the same, but quantitative differences were evidenced for lyso-PCs, which appear to be significantly more abundant in MPs.

Oxidation-Triggerable Liposome Incorporating Poly(Hydroxyethyl Acrylate-co-Allyl methyl sulfide) as an Anticancer Carrier of Doxorubicin

Fluorescence-activated cell sorting and confocal laser scanning microscopy revealed that doxorubicin (DOX)-loaded liposomes stabilized with folate-conjugated copolymers markedly promoted the transport of the anti-cancer drug to cancer cells.

Preparation of composites based in poly(3-hexylthiophene) and freeze-dried cellulose nanocrystals by a simple method, and their characterization

Poly(3-hexylthiophene) (P3HT)/cellulose nanocrystals (CNC) composites were prepared by a simple mixing method. The influence of the CNC incorporation on the properties of the composite was

Aspartic acid-based modified PLGA-PEG nanoparticles for bone targeting: in vitro and in vivo evaluation.

The Phospholipidomic Signatures of Human Blood Microparticles, Platelets and Platelet-Derived Microparticles: a Comparative HILIC-ESI–MS Investigation

The relative abundance of lyso-phosphatidylcholines among the eight identified phospholipid classes was found to be significantly higher in the lipid extracts of blood microparticles, and a higher relative incidence of species bearing polyunsaturated side chains, was observed in the case of bloodmicroparticles.




On the orientation of lipids in chloroplast and cell membranes

  • A. Benson
  • Biology, Chemistry
    Journal of the American Oil Chemists' Society
  • 1966
It is proposed that the lipids of membrane subunits are bound by hydrophobic association of the hydrocarbon chains regions within the interior of the protein, and the resulting two-dimensional lipoprotein aggregate would possess the strongly anionic charged groups of the phospholipids on its surface.

Preparation and Use of Liposomes as Models of Biological Membranes

The recognition that biological cells exploit the water-oil interfacial activity of certain lipids to define anatomical boundaries has, in recent years, encouraged many workers in this laboratory*

Plant Phospholipids and Glycolipids

Carbon-13 NMR studies of chloroplast membranes: carbon-13 enrichment without 13C-13C couplings.

  • R. Yashroy
  • Chemistry
    Journal of biochemical and biophysical methods
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A rapid method of total lipid extraction and purification.

The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.

Photosynthesis by isolated chloroplasts. IV. General concept and comparison of three photochemical reactions.

Methods in Membrane Biology

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    Springer US
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1 Electron Microscopic Methods in Membrane Biology.- 2 The Use of Phospholipases in the Determination of Asymmetric Phospholipid Distribution in Membranes.- 3 External Labeling of Cell Surface


This chapter discusses Photosynthesis and the Chloroplast, the Ultrastructure of Plastids, and Nucleic Acids and Information Processing in Chloroplasts, which aims to clarify the role of information storage and information processing in this complex system.

[Structure and function of chloroplasts].

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  • Environmental Science
    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
  • 1965
The chloroplast is a cell organelle that produces energy through photosynthesis, and has a high concentration of chlorophyll, the molecule that captures light energy, and this gives many plants and algae a green color.