Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy

@article{Yashroy2007LamellarDA,
  title={Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy},
  author={Rakesh Chander Yashroy},
  journal={Journal of Biosciences},
  year={2007},
  volume={15},
  pages={93-98}
}
  • R. C. Yashroy
  • Published 1 June 1990
  • Chemistry
  • Journal of Biosciences
Aqueous dispersions of lipids isolated from spinach chloroplast membranes were studied by electron microscopy after negative staining with phosphotungstic acid. Influence of low temperature (5°C for 24 h) was also investigated. It was observed that when contacted with water, these lipids, as such, formed multilamellar structures. Upon sonication, these multilamellar structures gave rise to a clear suspension of unilamellar vesicles varying in size (diameter) between 250 and 750 Å. When samples… 

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References

SHOWING 1-10 OF 17 REFERENCES

STRUCTURE AND ASSEMBLY OF MACROMOLECULAR LIPID COMPLEXES COMPOSED OF GLOBULAR MICELLES.

On the orientation of lipids in chloroplast and cell membranes

  • A. Benson
  • Biology, Chemistry
    Journal of the American Oil Chemists' Society
  • 1966
It is proposed that the lipids of membrane subunits are bound by hydrophobic association of the hydrocarbon chains regions within the interior of the protein, and the resulting two-dimensional lipoprotein aggregate would possess the strongly anionic charged groups of the phospholipids on its surface.

Preparation and Use of Liposomes as Models of Biological Membranes

The recognition that biological cells exploit the water-oil interfacial activity of certain lipids to define anatomical boundaries has, in recent years, encouraged many workers in this laboratory*

Plant Phospholipids and Glycolipids

Carbon-13 NMR studies of chloroplast membranes: carbon-13 enrichment without 13C-13C couplings.

  • R. Yashroy
  • Chemistry
    Journal of biochemical and biophysical methods
  • 1987

A rapid method of total lipid extraction and purification.

The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.

Photosynthesis by isolated chloroplasts. IV. General concept and comparison of three photochemical reactions.

Methods in Membrane Biology

  • E. Korn
  • Biology, Chemistry
    Springer US
  • 1976
1 Electron Microscopic Methods in Membrane Biology.- 2 The Use of Phospholipases in the Determination of Asymmetric Phospholipid Distribution in Membranes.- 3 External Labeling of Cell Surface

STRUCTURE AND FUNCTION OF CHLOROPLASTS

This chapter discusses Photosynthesis and the Chloroplast, the Ultrastructure of Plastids, and Nucleic Acids and Information Processing in Chloroplasts, which aims to clarify the role of information storage and information processing in this complex system.

[Structure and function of chloroplasts].

  • S. Murakami
  • Environmental Science
    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
  • 1965
The chloroplast is a cell organelle that produces energy through photosynthesis, and has a high concentration of chlorophyll, the molecule that captures light energy, and this gives many plants and algae a green color.