Lag-time measurement of antioxidant capacity using myoglobin and 2, 2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid): rationale, application, and limitation.

@article{Yu1999LagtimeMO,
  title={Lag-time measurement of antioxidant capacity using myoglobin and 2, 2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid): rationale, application, and limitation.},
  author={Ting Yu and Choon Nam Ong},
  journal={Analytical biochemistry},
  year={1999},
  volume={275 2},
  pages={217-23}
}
The application of a simple lag-time assay for antioxidant capacity using myoglobin and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) or ABTS has been studied for its general application conditions. In the presence of an antioxidant, the ABTS(*+) radical-cation-forming chromogenic reaction, catalyzed by myoglobin and initiated by hydrogen peroxide (H(2)O(2)), has a lag period, and its duration is linearly correlated to the concentration of that antioxidant. The high linearity between… CONTINUE READING

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