Lactose-induced expression of Bacillus sp. TS-23 amylase gene in Escherichia coli regulated by a T7 promoter.

@article{Lin1997LactoseinducedEO,
  title={Lactose-induced expression of Bacillus sp. TS-23 amylase gene in Escherichia coli regulated by a T7 promoter.},
  author={L L Lin and W H Hsu},
  journal={Letters in applied microbiology},
  year={1997},
  volume={24 5},
  pages={365-8}
}
Bacillus sp. TS-23 amylase gene was amplified by polymerase chain reaction and cloned into the pET23(+) transcription vector. Lactose induction of Escherichia coli BL21(DE3) cells harbouring this recombinant plasmid resulted in the extracellular production of gene products. By the addition of 20 mmol l-1 lactose, the amylase activity of fermentation broth… CONTINUE READING