Lactose-induced cell death of beta-galactosidase mutants in Kluyveromyces lactis.

@article{Lodi2005LactoseinducedCD,
  title={Lactose-induced cell death of beta-galactosidase mutants in Kluyveromyces lactis.},
  author={Tiziana Lodi and Claudia Donnini},
  journal={FEMS yeast research},
  year={2005},
  volume={5 8},
  pages={727-34}
}
The Kluyveromyces lactis lac4 mutants, lacking the beta-galactosidase gene, cannot assimilate lactose, but grow normally on many other carbon sources. However, when these carbon sources and lactose were simultaneously present in the growth media, the mutants were unable to grow. The effect of lactose was cytotoxic since the addition of lactose to an exponentially-growing culture resulted in 90% loss of viability of the lac4 cells. An osmotic stabilizing agent prevented cells killing, supporting… CONTINUE READING