Lactococcal proteinase maturation protein PrtM is a lipoprotein.

  title={Lactococcal proteinase maturation protein PrtM is a lipoprotein.},
  author={A. J. Haandrikman and Jan Kok and Gerard A. Venema},
  journal={Journal of bacteriology},
  volume={173 14},
The production of enzymatically active proteinase by lactococci requires the joint presence of a proteinase gene, prtP, and a gene encoding a maturation protein, prtM. A 32-kDa protein produced by Escherichia coli upon expression of the prtM gene under the direction of the T7 RNA polymerase promoter was purified and used to obtain PrtM-specific antibodies. With these antibodies, immunogold labeling of lactococcal cells revealed that PrtM was associated with the lactococcal cell envelope… CONTINUE READING
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