Lactobacillus plantarum WCFS1 β-Fructosidase: Evidence for an Open Funnel-Like Channel Through the Catalytic Domain with Importance for the Substrate Selectivity

  title={Lactobacillus plantarum WCFS1 $\beta$-Fructosidase: Evidence for an Open Funnel-Like Channel Through the Catalytic Domain with Importance for the Substrate Selectivity},
  author={Edgar Omar Mendoza-Llerenas and David J. P{\'e}rez and Zeferino G{\'o}mez-Sandoval and Pilar Escalante-Minakata and Vrani Ibarra-Junquera and Rodrigo Said Razo‐Hern{\'a}ndez and Vittorio Capozzi and Pasquale Russo and Giuseppe Spano and Daniela Fiocco and Juan A. Osuna‐Castro and Abel Moreno},
  journal={Applied Biochemistry and Biotechnology},
Abstractβ-Fructosidase, a glycoside hydrolase of a biotechnologically important strain, was studied for its biochemical, physicochemical, and three-dimensional structure characteristics. This enzyme was heterologously expressed in Escherichia coli as a C-terminal His-tagged protein (SacB). β-Fructosidase catalyzes the cleavage of glycoside bonds toward certain carbohydrates with β-fructofuranosyl linkages; however, SacB exhibited selectivity toward sucrose and an optimum activity at pH 6.0–6.5… Expand
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Cloning and Characterization of β-Fructosidase from Thermotoga neapolitana DSM4359 and Through the Degraded Glucoside
Research on degraded glycosides showed that β-fructosidase can act on sucrose and inulin to produce kestose and fructose–glucose syrup, and confirmed thatβ-fructose can tolerate high temperature and can be widely used in industrial production. Expand
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We solved the 1.8 Å crystal structure of β‐fructofuranosidase from Bifidobacterium longum KN29.1 – a unique enzyme that allows these probiotic bacteria to function in the human digestive system. TheExpand
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The X-ray structure of the enzyme:fructose complex, at a resolution of 1.87A, reveals two catalytically important residues: Asp41 and Glu241, a nucleophile and a catalytic acid/base, respectively, consistent with a double displacement mechanism of reaction. Expand
Cloning, expression and functional validation of a β-fructofuranosidase from Lactobacillus plantarum
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Three-dimensional Structure of Saccharomyces Invertase
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