Lactate-to-pyruvate or pyruvate-to-lactate assay for lactate dehydrogenase: a re-examination.

@article{Howell1979LactatetopyruvateOP,
  title={Lactate-to-pyruvate or pyruvate-to-lactate assay for lactate dehydrogenase: a re-examination.},
  author={B F Howell and Sylvia McCune and R D Schaffer},
  journal={Clinical chemistry},
  year={1979},
  volume={25 2},
  pages={269-72}
}
The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which allows more accurate spectrophotometric readout; (b) lower reactant concentrations are required, which substantially reduces the cost per assay; (c) solid reagents are used to… CONTINUE READING