Lack of sugar discrimination by human Pol mu requires a single glycine residue.

@article{Ruiz2003LackOS,
  title={Lack of sugar discrimination by human Pol mu requires a single glycine residue.},
  author={Jos{\'e} Javier Fern{\'a}ndez Ruiz and Raquel Ju{\'a}rez and Miguel Garc{\'i}a-D{\'i}az and Gloria Terrados and Angel J. Picher and Sergio Gonz{\'a}lez-Barrera and Antonio R. Fern{\'a}ndez de Henestrosa and Luis Blanco},
  journal={Nucleic acids research},
  year={2003},
  volume={31 15},
  pages={
          4441-9
        }
}
DNA polymerase mu (Pol mu) is a novel family X DNA polymerase that has been suggested to play a role in micro-homology mediated joining and repair of double strand breaks. We show here that human Pol mu is not able to discriminate against the 2'-OH group of the sugar moiety. It inserts rNTPs with an efficiency that is <10-fold lower than that of dNTPs, in sharp contrast with the >1000-fold discrimination characteristic of most DNA-dependent DNA polymerases. The lack of sugar discrimination by… CONTINUE READING

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CITES BACKGROUND

References

Publications referenced by this paper.
SHOWING 1-10 OF 31 REFERENCES

Crystal structure of 4448

M. Delarue, J. M. BouleÂ, +5 authors C. Papanicolaou
  • Nucleic Acids Research,
  • 2002
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

DNA polymerase l, a novel DNA repair enzyme in human cells

M. Garcõ Âa-Dõ Âaz, K. Bebenek, +9 authors L. Blanco
  • J. Biol. Chem.,
  • 2002
VIEW 1 EXCERPT

Lesion bypass activities of human DNA polymerase mu.

  • The Journal of biological chemistry
  • 2002
VIEW 1 EXCERPT