Lack of strong anti-viral immune gene stimulation in Torque Teno Sus Virus1 infected macrophage cells.

Abstract

While recent findings suggest that swine TTVs (TTSuVs) can act as primary or co-infecting pathogens, very little is known about viral immunity. To determine whether TTSuVs downregulate key host immune responses to facilitate their own survival, a swine macrophage cell line, 3D4/31, was used to over-express recombinant TTSuV1 viral particles or the ORF3 protein. Immune gene expression profiles were assessed by a quantitative PCR panel consisting of 22 immune genes, in cell samples collected at 6, 12, 24 and 48h post-transfection. Despite the upregulation of IFN-β and TLR9, interferon stimulated innate genes and pro-inflammatory genes were not upregulated in virally infected cells. The adaptive immune genes, IL-4 and IL-13, were significantly downregulated at 6h post-transfection. The ORF3 protein did not appear do not have a major immuno-suppressive effect, nor did it stimulate anti-viral immunity. Data from this study warrants further investigation into the mechanisms of TTV related immuno-pathogenesis.

DOI: 10.1016/j.virol.2016.04.028

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Cite this paper

@article{Singh2016LackOS, title={Lack of strong anti-viral immune gene stimulation in Torque Teno Sus Virus1 infected macrophage cells.}, author={Prim B . Singh and Sheela Ramamoorthy}, journal={Virology}, year={2016}, volume={495}, pages={63-70} }