Lack of inter-species reactivity between antigens and antibodies is overcome by protease treatment of western blots.

Abstract

The analysis of proteins by Western blotting is frequently limited by the inability of antibodies to recognize antigenic epitopes in proteins of different species. In the present study, we investigated the influence of mild protease digestion on the reactivity of nitrocellulose-blotted proteins and microscopic sections with antibodies produced against… (More)

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