Lack of complex I activity in human cells carrying a mutation in MtDNA-encoded ND4 subunit is corrected by the Saccharomyces cerevisiae NADH-quinone oxidoreductase (NDI1) gene.

@article{Bai2001LackOC,
  title={Lack of complex I activity in human cells carrying a mutation in MtDNA-encoded ND4 subunit is corrected by the Saccharomyces cerevisiae NADH-quinone oxidoreductase (NDI1) gene.},
  author={Yongli Bai and Petr H{\'a}jek and Anne Chomyn and Err-Cheng Chan and Byoung Boo Seo and Akemi Matsuno-Yagi and Toshiki Yagi and Giuseppe M. Attardi},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 42},
  pages={
          38808-13
        }
}
The gene for the single subunit, rotenone-insensitive, and flavone-sensitive internal NADH-quinone oxidoreductase of Saccharomyces cerevisiae (NDI1) can completely restore the NADH dehydrogenase activity in mutant human cells that lack the essential mitochondrial DNA (mtDNA)-encoded subunit ND4. In particular, the NDI1 gene was introduced into the nuclear genome of the human 143B.TK(-) cell line derivative C4T, which carries a homoplasmic frameshift mutation in the ND4 gene. Two transformants… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 45 CITATIONS

Obligatory role for complex I inhibition in the dopaminergic neurotoxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).

  • Toxicological sciences : an official journal of the Society of Toxicology
  • 2007
VIEW 4 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Mechanism of toxicity in rotenone models of Parkinson's disease.

  • The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 2003
VIEW 4 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED

References

Publications referenced by this paper.
SHOWING 1-3 OF 3 REFERENCES

Methods in Enzymology Mitochondrial Biogenesis and Genetics, Part B (Attardi, G., and Chomyn

A. Chomyn
  • A., eds)
  • 1996
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

Methods in Enzymology Mitochondrial Biogenesis and Genetics , Part B (

A. Chomyn
  • 1996

7187–7192 S. cerevisiae NDI1 Restores Complex I Activity in Human Cells

P. Bernardi, G. F. Azzone
  • J. Biol. Chem
  • 1981
VIEW 1 EXCERPT

Similar Papers

Loading similar papers…