Laboratory-scale method for enzymatic saccharification of lignocellulosic biomass at high-solids loadings

  title={Laboratory-scale method for enzymatic saccharification of lignocellulosic biomass at high-solids loadings},
  author={Christine M Roche and Clare J Dibble and Jonathan J. Stickel},
  booktitle={Biotechnology for biofuels},
BACKGROUND Screening new lignocellulosic biomass pretreatments and advanced enzyme systems at process relevant conditions is a key factor in the development of economically viable lignocellulosic ethanol. Shake flasks, the reaction vessel commonly used for screening enzymatic saccharifications of cellulosic biomass, do not provide adequate mixing at high-solids concentrations when shaking is not supplemented with hand mixing. RESULTS We identified roller bottle reactors (RBRs) as laboratory… CONTINUE READING

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