Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells.

@article{Clive1975LaboratoryPF,
  title={Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells.},
  author={D. Clive and J. Spector},
  journal={Mutation research},
  year={1975},
  volume={31 1},
  pages={
          17-29
        }
}
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References

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Specific-Locus Mutational Assay Systems for Mouse Lymphoma Cells
TLDR
Independent studies revealed that gene mutations are induced when Chinese hamster cells are incubated for short periods in the presence of alkylating agents and proved that the spontaneous mutation rate for mammalian tissue culture cells is actually several orders of magnitude lower than that assumed in Szybalski and Morrow's 1964 reports. Expand
A mutational assay system using the thymidine kinase locus in mouse lymphoma cells.
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An estimate can be made of the relative mutagenicities of various treatments of the TK locus and the observed induced mutation rates with the spontaneous TK +/− → TK −/− mutation rate. Expand
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Murine leukemic cells, L5178Y, inhibited by a low level of cytosine arabino-side for 6 hr could be rescued by deoxycytidine, and the incorporation into RNA could be correlated with irreversible inhibition of cell reproduction. Expand