Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells.

  title={Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells.},
  author={D. Clive and J. Spector},
  journal={Mutation research},
  volume={31 1},
Mutagenicity and genotoxicity studies of arruva, an R,R-monatin salt isomer.
Under the conditions of these studies, arruva was concluded to be negative in all three assays, thereby indicating the absence of its potential mutagenicity or genotoxicity under the conditions tested. Expand
Issues for conducting the microtiter version of the mouse lymphoma thymidine kinase (tk) assay and a critical review of data generated in a collaborative trial using the microtiter method.
The resulting data can be used as an exercise in data interpretation that will be helpful to both practitioners and regulators and provided a general framework for evaluating a series of mouse lymphoma experiments. Expand
In Vitro Mouse Lymphoma Cell (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay.
The assay when performed according to the standards recommended by the International Workshops on Genotoxicity Testing (IWGT) and the Organization of Economic Cooperation and Development Test Guideline 490 is capable of providing valuable genotoxicity hazard information as part of the overall safety assessment process of various classes of test substances. Expand
Cytotoxic activity of fucoxanthin, alone and in combination with the cancer drugs imatinib and doxorubicin, in CML cell lines.
The in vitro cytotoxicity of fucoxanthin (Fx) is unveiled by inhibition of cell proliferation in both cell lines, and the antiproliferative effects are not explained by induction of DNA damage or cell death. Expand
Genotoxic and mutagenic potential of camphorquinone in L5178/TK+/- mouse lymphoma cells.
CQ induced concentration-dependent, cytotoxic and genotoxic effects in L5178Y/TK+/- cells, most likely due to oxidative stress, but without mediating obvious biological relevant mutagenicity. Expand
Application of Evolving Computational and Biological Platforms for Chemical Safety Assessment
Abstract The 21st century is placing new demands on toxicology, such as the need to evaluate many more chemicals than ever before, and to make toxicity testing more relevant to human exposure.Expand
Chapter 6 – Genetic Toxicology Testing
The required genetic toxicity tests and several commonly used alternatives are described and the use of genetic toxicology throughout the drug development process from discovery through postmarketing is discussed. Expand
Critical review of the current literature on the safety of sucralose.
  • B. Magnuson, A. Roberts, E. Nestmann
  • Medicine
  • Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2017
Critical review of the extensive database of research demonstrates that sucralose is safe for its intended use as a non-caloric sugar alternative. Expand
Chapter 35 – Toxicity Methods
  • L. Di
  • Biology, Medicine
  • 2016
Resources used on toxicity methods are worthwhile to detect and measure toxic mechanisms to support prioritization and structure optimization and to reduce toxicity to a manageable level. Expand
FR-900098, an antimalarial development candidate that inhibits the non-mevalonate isoprenoid biosynthesis pathway, shows no evidence of acute toxicity and genotoxicity
It is concluded that FR-900098 lacks acute toxicity and genotoxicity, supporting its further development as an antimalarial drug. Expand


Specific-Locus Mutational Assay Systems for Mouse Lymphoma Cells
Independent studies revealed that gene mutations are induced when Chinese hamster cells are incubated for short periods in the presence of alkylating agents and proved that the spontaneous mutation rate for mammalian tissue culture cells is actually several orders of magnitude lower than that assumed in Szybalski and Morrow's 1964 reports. Expand
A mutational assay system using the thymidine kinase locus in mouse lymphoma cells.
An estimate can be made of the relative mutagenicities of various treatments of the TK locus and the observed induced mutation rates with the spontaneous TK +/− → TK −/− mutation rate. Expand
Mycoplasmas and cell cultures.
The incorporation of 3H-cytosine arabinoside and its effect on murine leukemic cells (L5178Y).
Murine leukemic cells, L5178Y, inhibited by a low level of cytosine arabino-side for 6 hr could be rescued by deoxycytidine, and the incorporation into RNA could be correlated with irreversible inhibition of cell reproduction. Expand