Laboratory evolution of cytochrome P450 BM‐3 monooxygenase for organic cosolvents

  title={Laboratory evolution of cytochrome P450 BM‐3 monooxygenase for organic cosolvents},
  author={Tuck Seng Wong and Frances H. Arnold and U. Schwaneberg},
  journal={Biotechnology and Bioengineering},
  • Tuck Seng Wong, Frances H. Arnold, U. Schwaneberg
  • Published 2004
  • Chemistry, Medicine
  • Biotechnology and Bioengineering
  • Cytochrome P450 BM‐3 (EC catalyzes the hydroxylation and/or epoxidation of a broad range of substrates, including alkanes, alkenes, alcohols, fatty acids, amides, polyaromatic hydrocarbons, and heterocycles. For many of these notoriously water‐insoluble compounds, P450 BM‐3's Km values are in the millimolar range. Polar organic cosolvents are therefore added to increase substrate solubility and achieve high catalytic efficiency. Using P450 BM‐3 as a catalyst for these important… CONTINUE READING
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