Microbiological analysis of discharge from the genitals in passenger car conductors (5787 women and 1496 men aged 20 to 40) showed Gardnerella vaginalis to be the most frequent agent of urogenital infection. It was isolated in 26% cases, whereas Trichomonas were isolated in 2.5%, fungi in 1.5%, and gonococci in 0.27% cases. The diagnosis of gardnerellosis is reliable if the key cells are found. Monoinfection with G. vaginalis was diagnosed in 80% patients, the overwhelming majority (74%) of carriers of this bacterium had no inflammatory symptoms, and in only 26% the carrier state was associated with the presence of leukocytes and histiocytes, more often in the cervix. In men the carrier state was detected in 0.4% cases. The clinical picture of Gardnerella infection is similar to that of infection with Mobiluncus, which is little known. This infection occurred 10 times less frequently (2.5%) than gardnerellosis, but in 92% cases it was a component of mixed infection, most frequently in association with G. vaginalis. A little known fungus Leptothrix was found in the genital discharge of 4% examinees, mostly women, but sometimes in men as well; it is represented by 3 types of ramifying threads. The practitioners are to know these infections causing specific diseases, such as bacterial vaginosis, which are often responsible for serious complications in gynecology and obstetrics.