Laboratory Methods in Molecular Epidemiology: Bacterial Infections.

@article{Riley2018LaboratoryMI,
  title={Laboratory Methods in Molecular Epidemiology: Bacterial Infections.},
  author={Lee W. Riley},
  journal={Microbiology spectrum},
  year={2018},
  volume={6 6}
}
  • L. Riley
  • Published 2 November 2018
  • Medicine, Biology
  • Microbiology spectrum
In infectious disease epidemiology, the laboratory plays a critical role in diagnosis, outbreak investigations, surveillance, and characterizing biologic properties of microbes associated with their transmissibility, resistance to anti-infectives, and pathogenesis. The laboratory can inform and refine epidemiologic study design and data analyses. In public health, the laboratory functions to assess effect of an intervention. In addition to research laboratories, the new-generation molecular… 
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References

SHOWING 1-10 OF 154 REFERENCES
Overview of molecular typing methods for outbreak detection and epidemiological surveillance.
  • A. Sabat, A. Budimir, A. Friedrich
  • Biology, Medicine
    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
  • 2013
TLDR
Current and new molecular typing methods for outbreak detection and epidemiological surveillance of bacterial pathogens in clinical practice are reviewed to give an overview of their specific advantages and disadvantages.
Impacts of culture-independent diagnostic practices on public health surveillance for bacterial enteric pathogens.
  • A. Cronquist, R. Mody, B. Mahon
  • Medicine, Biology
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 2012
TLDR
The challenges and opportunities that culture-independent tests present are summarized and strategies, such as validation studies and development of culture- independent tests compatible with subtyping, that could be adopted are suggested to ensure that surveillance remains robust.
Studying bacterial infections through culture-independent approaches.
TLDR
It is proposed that at all locations within the human body where infection is routinely initiated within the context of a community of microbes, the same principles will apply.
Molecular Epidemiology of Infectious Diseases
TLDR
Molecular Epidemiology of Infectious Diseases: Principles and Practices outlines the principles, methods, and applications of this exciting new discipline of public health, preparing health researchers, practitioners, and students to address globally and locally the problems associated with infectious diseases.
How to select and interpret molecular strain typing methods for epidemiological studies of bacterial infections: a review for healthcare epidemiologists. Molecular Typing Working Group of the Society for Healthcare Epidemiology of America.
TLDR
Strain typing cannot be used to replace a sound epidemiological investigation, but serves as a useful adjunct to such investigations, and criteria to aid epidemiologists in interpreting results have been published.
Application of Whole-Genome Sequencing for Bacterial Strain Typing in Molecular Epidemiology
TLDR
It is demonstrated that PFGE is prone to false-positive and false-negative results and suggests the need for a new gold standard approach for molecular epidemiological strain typing, and bacterial whole-genome sequencing (WGS) is explored as an alternative approach for Molecular strain typing.
Manual of Clinical Microbiology, 11th Edition
TLDR
There are some minor inaccuracies in a number of the subchapters throughout the book, but the impact of these and other inaccuracies on the reading experience as a whole, is minor.
Pulsed field gel electrophoresis: a review of application and interpretation in the molecular epidemiology of infectious disease.
  • R. Goering
  • Biology
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • 2010
Multilocus sequence typing of bacteria.
  • M. Maiden
  • Biology
    Annual review of microbiology
  • 2006
Multilocus sequence typing (MLST) was proposed in 1998 as a portable, universal, and definitive method for characterizing bacteria, using the human pathogen Neisseria meningitidis as an example. In
Large-scale genomic sequencing of extraintestinal pathogenic Escherichia coli strains.
TLDR
This study reveals what kind of information can be obtained from sequencing clinical isolates on a large scale, even well-characterized organisms such as E. coli, and provides insight into how this information might be utilized in a healthcare setting.
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