Labelling quality and chromosome morphology after low temperature FISH analysed by scanning far-field and near-field optical microscopy.

@article{Winkler2003LabellingQA,
  title={Labelling quality and chromosome morphology after low temperature FISH analysed by scanning far-field and near-field optical microscopy.},
  author={Ralph Winkler and Birgit Perner and Alexander Rapp and M E Durm and Christoph Cremer and K Greulich and Michael Hausmann},
  journal={Journal of microscopy},
  year={2003},
  volume={209 Pt 1},
  pages={23-33}
}
A non-enzymatic, low temperature fluorescence in situ hybridization (LTFISH) procedure was applied to metaphase spreads and interphase cell nuclei. In this context 'low temperature' means that the denaturation procedure of the chromosomal target DNA usually applied by heat treatment and chaotropic agents such as formamide was completely omitted so that the complete hybridization reaction took place at 37 degrees C. For LTFISH, the DNA probe had to be single-stranded, which was achieved by means… CONTINUE READING

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