Labelled‐replica techniques: post‐shadow labelling of intramembrane particles in freeze‐fracture replicas

@article{Rash1982LabelledreplicaTP,
  title={Labelled‐replica techniques: post‐shadow labelling of intramembrane particles in freeze‐fracture replicas},
  author={John E Rash and Timothy J. A. Johnson and C. Sue Hudson and F D Giddings and W F Graham and Mohyee E. Eldefrawi},
  journal={Journal of Microscopy},
  year={1982},
  volume={128}
}
Three methods are described for direct post‐fracture, post‐shadow labelling of individual classes of intramembrane particles (IMPs) in freeze‐fracture replicas of biological membranes. The P‐face IMPs corresponding to the acetylcholine receptor complexes (AChRs) of vertebrate neuroeffector junctions are identified by post‐replication labelling with ferritin‐antibody complexes and with neurotoxin‐biotin‐avidin‐colloidal gold affinity ligands. (The freeze‐etch nomenclature of Branton et al., 1975… Expand
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TLDR
This chapter discusses the assays involving freeze-fracture and freeze-etching, which permits the study of biological structures “fixed” only by freezing, thus circumventing a number of artifacts linked to other preparation methods for electron microscopy. Expand
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  • Chemistry, Medicine
  • Journal of electron microscopy technique
  • 1989
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Publisher Summary This chapter provides information regarding specimen preparation, the fracture/replicating process, and interpretation of freeze-fracture replicas. The methodology described in theExpand
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