Labelled‐replica techniques: post‐shadow labelling of intramembrane particles in freeze‐fracture replicas

@article{Rash1982LabelledreplicaTP,
  title={Labelled‐replica techniques: post‐shadow labelling of intramembrane particles in freeze‐fracture replicas},
  author={J. Rash and T. J. Johnson and C. Hudson and F. Giddings and W. F. Graham and M. Eldefrawi},
  journal={Journal of Microscopy},
  year={1982},
  volume={128}
}
  • J. Rash, T. J. Johnson, +3 authors M. Eldefrawi
  • Published 1982
  • Chemistry, Medicine
  • Journal of Microscopy
  • Three methods are described for direct post‐fracture, post‐shadow labelling of individual classes of intramembrane particles (IMPs) in freeze‐fracture replicas of biological membranes. The P‐face IMPs corresponding to the acetylcholine receptor complexes (AChRs) of vertebrate neuroeffector junctions are identified by post‐replication labelling with ferritin‐antibody complexes and with neurotoxin‐biotin‐avidin‐colloidal gold affinity ligands. (The freeze‐etch nomenclature of Branton et al., 1975… CONTINUE READING
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    References

    SHOWING 1-10 OF 86 REFERENCES
    Fracture-label:O cytochemistry of freeze-fracture faces in the erythrocyte membrane.
    • 54
    • PDF
    THE STRUCTURE OF ERYTHROCYTE MEMBRANES STUDIED BY FREEZE-ETCHING
    • 262
    • PDF
    Prefracture and cold-fracture images of yeast plasma membranes
    • 27
    • PDF
    A freeze-etch study of the effects of filipin on liposomes and human erythrocyte membranes.
    • 116
    Freeze-fracture autoradiography: feasibility
    • 30
    • PDF
    Freeze fracture studies of human neuromuscular junctions. Membrane alterations observed in myasthenia gravis.
    • 15