Labelled‐replica techniques: post‐shadow labelling of intramembrane particles in freeze‐fracture replicas

@article{Rash1982LabelledreplicaTP,
  title={Labelled‐replica techniques: post‐shadow labelling of intramembrane particles in freeze‐fracture replicas},
  author={John E Rash and Timothy J. A. Johnson and C. Sue Hudson and F D Giddings and W F Graham and Mohyee E. Eldefrawi},
  journal={Journal of Microscopy},
  year={1982},
  volume={128}
}
Three methods are described for direct post‐fracture, post‐shadow labelling of individual classes of intramembrane particles (IMPs) in freeze‐fracture replicas of biological membranes. The P‐face IMPs corresponding to the acetylcholine receptor complexes (AChRs) of vertebrate neuroeffector junctions are identified by post‐replication labelling with ferritin‐antibody complexes and with neurotoxin‐biotin‐avidin‐colloidal gold affinity ligands. (The freeze‐etch nomenclature of Branton et al., 1975… 

Composite replicas: methodologies for direct evaluation of the relationship between intramembrane and extramembrane structures.

While some unlabelled or lightly labelled surface elements can be lost from fractured membranes, ligands stabilize elements and reduce their loss apparently by cross-linking them.

Postreplication labeling of E-leaflet molecules: membrane immunoglobulins localized in sectioned, labeled replicas examined by TEM and HVEM.

Postreplication E-surface labeling of split and replicated membranes is a high-resolution technique that is suitable for the study of membrane protein distribution in E-face replicas and contiguous nonreplicated tissue.

Freeze‐fracture cytochemistry: a simplified guide and update on developments

Recent developments have centred on the use of replicas to stabilize half‐membrane leaflets; not only may these and associated attached components be retained for labelling just before mounting, but they provide a means for manipulating the specimen— specifically, turning it over during processing—so that additional structural information can be obtained.

Label-fracture: a method for high resolution labeling of cell surfaces

"Label-fracture" provides extensive views of the distribution of the label on membrane surfaces while preserving cell shape and relating to the freeze-f Fracture morphology of exoplasmic fracture faces.

Freeze-fracture cytochemistry: review of methods.

  • N. Severs
  • Biology
    Journal of electron microscopy technique
  • 1989
The purpose of this review is to present a systematic survey of the full range of techniques currently available in freeze-fracture cytochemistry, with emphasis on explaining the principles underlying the methods and on illustrating their applications.

Freeze—fracture and freeze—etch electron microscopy of membrane proteins

Using freeze—fracturing, investigators have visualized a broad spectrum of membrane morphologies that have served to refine and change earlier concepts of membrane structure, particularly with respect to the organization of membrane proteins.

(Na ÷ + K÷)-ATPase Correlated of Intramembrane Particles in of Cultured Chick Myotubes with a Major Group Freeze-Fracture Replicas

It is concluded that the intramembrane particles patched by double antibody cross-linkage of the ( Na, K)-ATPase are caused by (Na, K) ATPase molecules in the fracture plane.

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