Labeling tetracysteine-tagged proteins with biarsenical dyes for live cell imaging.

Abstract

Correlation of real-time or time-lapse light microscopy (LM) with electron microscopy (EM) of cells can be performed with biarsenical dyes. These dyes fluorescently label tetracysteine-tagged proteins so that they can be imaged with LM and, upon fluorescent photoconversion of 3,3'-diaminobenzidine tetrahydrochloride (DAB), with EM as well. In the following… (More)
DOI: 10.1101/pdb.prot5547

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