Labeling surface proteins with high specificity: Intrinsic limitations of phosphopantetheinyl transferase systems

@article{Stber2019LabelingSP,
  title={Labeling surface proteins with high specificity: Intrinsic limitations of phosphopantetheinyl transferase systems},
  author={Jakob C St{\"u}ber and A. Pl{\"u}ckthun},
  journal={PLoS ONE},
  year={2019},
  volume={14}
}
  • Jakob C Stüber, A. Plückthun
  • Published 2019
  • Chemistry, Medicine
  • PLoS ONE
  • Objective Fluorescent labeling of specific cell-surface proteins enables a manifold of techniques to study their function in health and disease. A frequently cited family of methods employs phosphopantetheinyl transferases (PPTases) to attach probes, provided as conjugates of Coenzyme A. This method appears attractive, as only short peptide tags genetically fused to the protein of interest are needed as conjugation sites. Here, we describe observations we made when evaluating such protocols for… CONTINUE READING
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    References

    SHOWING 1-10 OF 37 REFERENCES
    Specific labeling of cell surface proteins with chemically diverse compounds.
    • 262
    • Highly Influential
    HaloTag: a novel protein labeling technology for cell imaging and protein analysis.
    • 1,179
    • Highly Influential
    • PDF
    Labeling proteins with small molecules by site-specific posttranslational modification.
    • 164
    • PDF
    Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases.
    • 170
    • Highly Influential
    • PDF
    Tag-probe labeling methods for live-cell imaging of membrane proteins.
    • 43
    • PDF
    Fluorophore targeting to cellular proteins via enzyme-mediated azide ligation and strain-promoted cycloaddition.
    • 97
    • PDF
    Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase.
    • Jun Yin, P. Straight, +6 authors C. Walsh
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 2005
    • 295
    • PDF
    How to obtain labeled proteins and what to do with them.
    • 224
    • PDF