Labeling of specific cysteines in proteins using reversible metal protection.

@article{Puljung2011LabelingOS,
  title={Labeling of specific cysteines in proteins using reversible metal protection.},
  author={Michael C Puljung and William N Zagotta},
  journal={Biophysical journal},
  year={2011},
  volume={100 10},
  pages={2513-21}
}
Fluorescence spectroscopy is an indispensible tool for studying the structure and conformational dynamics of protein molecules both in isolation and in their cellular context. The ideal probes for monitoring intramolecular protein motions are small, cysteine-reactive fluorophores. However, it can be difficult to obtain specific labeling of a desired cysteine in proteins with multiple cysteines, in a mixture of proteins, or in a protein's native environment, in which many cysteine-containing… CONTINUE READING