Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles

@article{Pittet2006LabelingOI,
  title={Labeling of immune cells for in vivo imaging using magnetofluorescent nanoparticles},
  author={Mikael J. Pittet and Filip K. Swirski and Fred Reynolds and Lee Josephson and Ralph Weissleder},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={73-79}
}
Observation of immune and stem cells in their native microenvironments requires the development of imaging agents to allow their in vivo tracking. We describe here the synthesis of magnetofluorescent nanoparticles for cell labeling in vitro and for multimodality imaging of administered cells in vivo. MION-47, a prototype monocrystalline iron oxide nanoparticle, was first converted to an intermediate bearing a fluorochrome and amine groups, then reacted with either HIV-Tat peptide or protamine… 
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