Labeling of dendritic spines with the carbocyanine dye DiI for confocal microscopic imaging in lightly fixed cortical slices.

@article{Kim2007LabelingOD,
  title={Labeling of dendritic spines with the carbocyanine dye DiI for confocal microscopic imaging in lightly fixed cortical slices.},
  author={Byung Gook Kim and Hai-ning Dai and Marietta McAtee and Stefano Vicini and Barbara S. Bregman},
  journal={Journal of neuroscience methods},
  year={2007},
  volume={162 1-2},
  pages={237-43}
}
Visualization of dendritic spines is an important tool for researches on structural synaptic plasticity. Fluorescent labeling of the dendrites and spines followed by confocal microscopy permits imaging a large population of dendritic spines with a higher resolution. We sought to establish an optimal protocol to label neurons in cortical slices with the carbocyanine dye DiI for confocal microscopic imaging of dendritic spines. DiI finely labeled dendrites and spines in slices prefixed (by… CONTINUE READING
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