Labeling of an antisense oligonucleotide with [(18)F]FPy5yne.

  title={Labeling of an antisense oligonucleotide with [(18)F]FPy5yne.},
  author={James A. H. Inkster and Michael J. Adam and Tim Storr and Thomas J. Ruth},
  journal={Nucleosides, nucleotides & nucleic acids},
  volume={28 11},
Functional imaging of gene expression in vivo with short-lived positron emitter (18)F remains an unrealized goal, in part because the long reaction times and challenging protocols typically required to label nucleic acid-based molecular probes with this radionuclide (t(1/2) = 109.8 minutes). To this end, we synthesized prosthetic group 2-[(18)F]fluoro-3-(hex-5-ynyloxy)pyridine ([(18)F]FPy5yne), used previously to label peptides, and coupled it to an oligodeoxyribonucleotide with (18)F by way of… CONTINUE READING