Labeling lysine acetyltransferase substrates with engineered enzymes and functionalized cofactor surrogates.

@article{Yang2013LabelingLA,
  title={Labeling lysine acetyltransferase substrates with engineered enzymes and functionalized cofactor surrogates.},
  author={Chao Yang and Jiaqi Mi and You Feng and Liza D. Ngo and Tielong Gao and Leilei Yan and Yujun George Zheng},
  journal={Journal of the American Chemical Society},
  year={2013},
  volume={135 21},
  pages={7791-4}
}
Elucidating biological and pathological functions of protein lysine acetyltransferases (KATs) greatly depends on the knowledge of the dynamic and spatial localization of their enzymatic targets in the cellular proteome. We report the design and application of chemical probes for facile labeling and detection of substrates of the three major human KAT enzymes. In this approach, we create engineered KATs in junction with synthetic Ac-CoA surrogates to effectively label KAT substrates even in the… CONTINUE READING