Label-free quantification in ion mobility–enhanced data-independent acquisition proteomics

@article{Distler2016LabelfreeQI,
  title={Label-free quantification in ion mobility–enhanced data-independent acquisition proteomics},
  author={Ute Distler and J{\"o}rg Kuharev and Pedro Navarro and Stefan Tenzer},
  journal={Nature Protocols},
  year={2016},
  volume={11},
  pages={795-812}
}
Unbiased data-independent acquisition (DIA) strategies have gained increased popularity in the field of quantitative proteomics. The integration of ion mobility separation (IMS) into DIA workflows provides an additional dimension of separation to liquid chromatography-mass spectrometry (LC-MS), and it increases the achievable analytical depth of DIA approaches. Here we provide a detailed protocol for a label-free quantitative proteomics workflow based on ion mobility–enhanced DIA, which… CONTINUE READING
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