Label-free protein quantitation using weighted spectral counting.

@article{Vogel2012LabelfreePQ,
  title={Label-free protein quantitation using weighted spectral counting.},
  author={Christine Vogel and Edward M. Marcotte},
  journal={Methods in molecular biology},
  year={2012},
  volume={893},
  pages={321-41}
}
Mass spectrometry (MS)-based shotgun proteomics allows protein identifications even in complex biological samples. Protein abundances can then be estimated from the counts of MS/MS spectra attributable to each protein, provided that one corrects for differential MS-detectability of the contributing peptides. We describe the use of a method, APEX, which calculates Absolute Protein EXpression levels based on learned correction factors, MS/MS spectral counts, and each protein's probability of… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 50 references

Similar Papers

Loading similar papers…