Label-free protein quantification using LC-coupled ion trap or FT mass spectrometry: Reproducibility, linearity, and application with complex proteomes.

@article{Wang2006LabelfreePQ,
  title={Label-free protein quantification using LC-coupled ion trap or FT mass spectrometry: Reproducibility, linearity, and application with complex proteomes.},
  author={Guanghui Wang and Wells W. Wu and Weihua S Zeng and C L Chou and Rong-Fong Shen},
  journal={Journal of proteome research},
  year={2006},
  volume={5 5},
  pages={1214-23}
}
A critical step in protein biomarker discovery is the ability to contrast proteomes, a process referred generally as quantitative proteomics. While stable-isotope labeling (e.g., ICAT, 18O- or 15N-labeling, or AQUA) remains the core technology used in mass spectrometry-based proteomic quantification, increasing efforts have been directed to the label-free approach that relies on direct comparison of peptide peak areas between LC-MS runs. This latter approach is attractive to investigators for… CONTINUE READING

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