Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy

  title={Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy},
  author={Tsubasa Kobayashi and Yu-Rok Cha and Yuichi Kaji and Tetsuro Oshika and Philippe Leproux and Vincent Couderc and Hideaki Kano},
Acanthamoeba keratitis is a disease in which amoebae named Acanthamoeba invade the cornea of an eye. To diagnose this disease before it becomes serious, it is important to detect the cyst state of Acanthamoeba in the early stage of infection. In the present study, we explored spectroscopic signitures of the cyst state of Acanthamoeba using multimodal nonlinear optical microscopy with the channels of multiplex coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and… 


SHG-specificity of cellular Rootletin filaments enables naïve imaging with universal conservation
Rootletin filaments in rootlets in the second harmonic generation channel were visualized in custom coherent nonlinear optical microscopy with a simple, compact, and ultra-broadband supercontinuum light source and it was confirmed that similar fibrous SHG was observed even in unicellular ciliates.
Acanthamoeba spp. as Agents of Disease in Humans
Since an increase in the number of cases of Acanthamoeba infections has occurred worldwide, these protozoa have become increasingly important as agents of human disease.
On the origin of the 1602 cm–1 Raman band of yeasts; contribution of ergosterol
It is suggested that the 1602 cm(-1) Raman signature could serve as an intrinsic ergosterol marker in yeasts for the study of sterol metabolism in vivo and in a label-free manner, which could not be done by any other techniques at the current stage.
Quantitative CARS molecular fingerprinting of single living cells with the use of the maximum entropy method.
The authors thank C. Onogi for providing the spontaneous Raman spectrum of yeast mitochondria, Leukos and Horus Laser companies for technical support, and Dr. Yomo (Suntory Co., Ltd.) for providing us with the yeast sample.
Direct extraction of Raman line-shapes from congested CARS spectra.
It is shown that Raman line-shapes can be extracted directly from congested coherent anti-Stokes Raman scattering (CARS) spectra, by using a numerical method to retrieve the phase-information hidden in measured CARS spectra using the maximum entropy (ME) model.
The master programme in Applied Geology aims to provide comprehensive knowledge based on various branches of Geology, with special focus on Applied geology subjects in the areas of Geomorphology, Structural geology, Hydrogeology, Petroleum Geologists, Mining Geology), Remote Sensing and Environmental geology.
  • Leproux et al., “Quantitative CARS Molecular Fingerprinting of Single Living Cells with the Use of the Maximum Entropy Method,” Angewandte Chemie International Edition, 49(38), 6773-6777
  • 2010
  • Kaji et al., “SHG-specificity of cellular Rootletin filaments enables naive imaging with universal conservation,” Scientific reports, 7, 39967
  • 2017
Acanthamoeba spp
  • as Agents of Disease in Humans,” Clinical Microbiology Reviews, 16(2), 273-307
  • 2003