Label-free evanescent microscopy for membrane nano-tomography in living cells.

Abstract

We show that through-the-objective evanescent microscopy (epi-EM) is a powerful technique to image membranes in living cells. Readily implementable on a standard inverted microscope, this technique enables full-field and real-time tracking of membrane processes without labeling and thus signal fading. In addition, we demonstrate that the membrane/interface… (More)
DOI: 10.1002/jbio.201300095

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