Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation

  title={Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation},
  author={Li Xia Gao and Chunxiang Bian and Yan Wu and Muhammad Farrukh Nisar and Shida Chen and Chang Ming Li and Ling Yu and Ping Ji and Enyi Huang and Julia Li Zhong},
  journal={Journal of Biological Engineering},
BackgroundGeneration of reactive oxygen species (ROS), triggered by ultraviolet radiation (UVR), is associated with carcinogenesis of the skin. UV irradiation induced superoxide anion (O2•−) is the key ROS involved in the cellular damage. The cytoprotective efficacy of an unknown anti-oxidant compound can be evaluated by analyzing the production of O2•− from treated cells.MethodsIn this study, a glass carbon electrode functionalized with nanotube@DNA-Mn3(PO4)2 composite was applied to… 
3 Citations

Alpha-Tocopherol Protects Human Dermal Fibroblasts by Modulating Nitric Oxide Release, Mitochondrial Function, Redox Status, and Inflammation

Background: The altered balance between oxidants/antioxidants and inflammation, changes in nitric oxide (NO) release, and mitochondrial function have a role in skin aging through fibroblast

Reactive oxygen species in the biological system: challenges and methodological advances

The study presented here emphasize recent development on in-vivo detection and visualization of oxidative stress using various techniques and add the contribution to the development of an electrochemical biosensor based on catalytic amperometry to simultaneously monitor oxygen consumption and hydrogen peroxide production in real-time.



Scavenging or Quenching Effect of Melanin on Superoxide Anion and Singlet Oxygen

It is confirmed that one of the mechanisms by which melanin protects UV-induced skin damage is likely scavenging or quenching activity against ROS such as O2•− and 1O2.

Effect of alpha-tocopherol on cytotoxicity induced by UV irradiation and antioxidants.

The present study suggests that the protective effect of alpha-tocopherol might be derived from its incorporation into the cell membranes rather than its scavenging activity.

Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin.

Nrf2- and Bach1 May Play a Role in the Modulation of Ultraviolet A-Induced Oxidative Stress by Acetyl-11-Keto-β-Boswellic Acid in Skin Keratinocytes

The cytoprotective effects of AKBA against UVA-derived oxidative damage were accompanied by modulating expression of inflammatory mediators and NOX1, and therefore, AKBA has potential in the development of skin care products.


Results are consistent with the conclusion that the generation of singlet oxygen, but not hydrogen peroxide or hydroxyl radical, plays an important role in the inactivation of cultured human cells by UVA and near‐visible radiations.

Irradiation of skin with visible light induces reactive oxygen species and matrix-degrading enzymes.

Findings suggest that other portions of the solar spectrum aside from UV, particularly visible light, may also contribute to signs of premature photoaging in skin.

Modulation of gene expression by the oxidative stress generated in human skin cells by UVA radiation and the restoration of redox homeostasis

  • R. Tyrrell
  • Biology, Chemistry
    Photochemical & Photobiological Sciences
  • 2012
Levels of both antioxidant and pro-oxidant proteins, including manganese-dependent superoxide dismutase, glutathione peroxidase, hemeoxygenase 1, NADPH oxidase, ferritin, and methionine-S-sulfoxidereductase, are increased by UVA treatment and following moderate dose levels these will contribute to either the restoration or a further perturbation of redox homeostasis.

The anti-apoptotic and anti-oxidant effect of eriodictyol on UV-induced apoptosis in keratinocytes.

Findings suggest that eriodictyol can be used to protect keratinocytes from UV-induced damage, implying the presence of a complex structure-activity relationship (SAR) in the differential apoptosis-modulating activities of various flavonoids.