Label-free detection of microRNA based on the fluorescence quenching of silicon nanoparticles induced by catalyzed hairpin assembly coupled with hybridization chain reaction

@article{Ding2018LabelfreeDO,
  title={Label-free detection of microRNA based on the fluorescence quenching of silicon nanoparticles induced by catalyzed hairpin assembly coupled with hybridization chain reaction},
  author={Longhua Ding and Haiyun Liu and Lina Zhang and Li Li and Jinghua Yu},
  journal={Sensors and Actuators B-chemical},
  year={2018},
  volume={254},
  pages={370-376}
}
Abstract MicroRNAs (miRNAs) play important roles in the regulation of cellular activities and are considered as potential biomarkers for various diseases. The detection of miRNA has become a highly important aspect for clinical diagnostics. In this work, a simple method for detection of miRNA is developed based on the fluorescence quenching of silicon nanoparticles (SiNPs) induced by the inner filter effects (IFE). The target miRNA triggers the hybridization chain reaction (HCR) of two DNA… Expand
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