Label-free detection and identification of protein ligands captured by receptors in a polymerized planar lipid bilayer using MALDI-TOF MS

  title={Label-free detection and identification of protein ligands captured by receptors in a polymerized planar lipid bilayer using MALDI-TOF MS},
  author={Boying Liang and Yue Ju and James R Joubert and Erin J. Kaleta and Rodrigo Lopez and Ian W. Jones and H. K. Jr. Hall and Saliya N Ratnayaka and Vicki H. Wysocki and S Scott Saavedra},
  journal={Analytical and Bioanalytical Chemistry},
AbstractMatrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) coupled with affinity capture is a well-established method to extract biological analytes from complex samples followed by label-free detection and identification. Many bioanalytes of interest bind to membrane-associated receptors; however, the matrices and high-vacuum conditions inherent to MALDI-TOF MS make it largely incompatible with the use of artificial lipid membranes with incorporated… Expand
3 Citations
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