Label-free comparative analysis of proteomics mixtures using chromatographic alignment of high-resolution muLC-MS data.

@article{Finney2008LabelfreeCA,
  title={Label-free comparative analysis of proteomics mixtures using chromatographic alignment of high-resolution muLC-MS data.},
  author={Gregory L. Finney and Adele Rae Blackler and Michael R. Hoopmann and Jesse D. Canterbury and Christine C. Wu and Michael J. MacCoss},
  journal={Analytical chemistry},
  year={2008},
  volume={80 4},
  pages={
          961-71
        }
}
Label-free relative quantitative proteomics is a powerful tool for the survey of protein level changes between two biological samples. We have developed and applied an algorithm using chromatographic alignment of microLC-MS runs to improve the detection of differences between complex protein mixtures. We demonstrate the performance of our software by finding differences in E. coli protein abundance upon induction of the lac operon genes using isopropyl beta-D-thiogalactopyranoside. The use of… CONTINUE READING
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