Label-free and non-enzymatic detection of DNA based on hybridization chain reaction amplification and dsDNA-templated copper nanoparticles.

@article{Song2014LabelfreeAN,
  title={Label-free and non-enzymatic detection of DNA based on hybridization chain reaction amplification and dsDNA-templated copper nanoparticles.},
  author={Chunxia Song and Xiaohai Yang and Qing Wang and Jin Huang and Jianbo Liu and Wenhui Liu and Pei Liu},
  journal={Analytica chimica acta},
  year={2014},
  volume={827},
  pages={
          74-9
        }
}
A label-free and non-enzymatic amplification fluorescent method for detection of DNA has been developed by using hybridization chain reaction (HCR) and dsDNA-templated copper nanoparticles (CuNPs). First, the biotinylated capture DNA probes were immobilized on the streptavidin-modified beads through the interaction of biotin and streptavidin. Then, target DNA hybridized with the capture DNA probes, which formed a hybridized DNA with sticky end. The sticky end triggered the HCR process and… CONTINUE READING

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