Label-Free and Sensitive Fluorescent Detection of Sequence-Specific Single-Strand DNA Based on S1 Nuclease Cleavage Effects

@inproceedings{Guan2014LabelFreeAS,
  title={Label-Free and Sensitive Fluorescent Detection of Sequence-Specific Single-Strand DNA Based on S1 Nuclease Cleavage Effects},
  author={Zheng Guan and Jinchuan Liu and Wenhui Bai and Zhenzhen Lv and Xiaoling Jiang and Shuming Yang and Ailiang Chen and Guiyuan Lv and Albert Jeltsch},
  booktitle={PloS one},
  year={2014}
}
The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic. Here, we have demonstrated a simple label-free sensing strategy to detect ssDNA by employing its complementary ssDNA, S1 nuclease and nucleic acid fluorescent dyes. Upon clearing away redundant complementary ssDNA and possibly mismatched double… CONTINUE READING