LPS-induced depolymerization of cytoskeleton and its role in TNF-α production by rat pneumocytes.

@article{Isowa1999LPSinducedDO,
  title={LPS-induced depolymerization of cytoskeleton and its role in TNF-α production by rat pneumocytes.},
  author={Noritaka Isowa and Alexandre Martins Xavier and Ewa Dziak and M. Opas and Donna I McRitchie and Arthur S Slutsky and Shaf H Keshavjee and Mingyao Liu},
  journal={The American journal of physiology},
  year={1999},
  volume={277 3 Pt 1},
  pages={L606-15}
}
Lipopolysaccharide (LPS) polymerizes microfilaments and microtubules in macrophages and monocytes. Disrupting microfilaments or microtubules with cytochalasin D (CytoD) or colchicine can suppress LPS-induced tumor necrosis factor-α (TNF-α) gene expression and protein production from these cells. We have recently demonstrated that primary cultured rat alveolar epithelial cells can produce TNF-α on LPS stimulation. In the present study, we found that the LPS-induced increase in TNF-α mRNA level… CONTINUE READING