LOVTRAP, An Optogenetic System for Photo-induced Protein Dissociation

  title={LOVTRAP, An Optogenetic System for Photo-induced Protein Dissociation},
  author={Hui Wang and Marco Vilela and Andreas Winkler and Miroslaw Tarnawski and Ilme Schlichting and Hayretin Yumerefendi and Brian Kuhlman and Rihe Liu and Gaudenz Danuser and Klaus Michael Hahn},
  booktitle={Nature Methods},
LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins. 
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