LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells.

@article{Ravesteyn2016LNAMO,
  title={LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells.},
  author={Thomas W van Ravesteyn and Marleen Dekker and Alexander Fish and Titia Sixma and Astrid Wolters and Rob J. Dekker and Hein Te Riele},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2016},
  volume={113 15},
  pages={4122-7}
}
Synthetic single-stranded DNA oligonucleotides (ssODNs) can be used to generate subtle genetic modifications in eukaryotic and prokaryotic cells without the requirement for prior generation of DNA double-stranded breaks. However, DNA mismatch repair (MMR) suppresses the efficiency of gene modification by >100-fold. Here we present a commercially available ssODN design that evades MMR and enables subtle gene modification in MMR-proficient cells. The presence of locked nucleic acids (LNAs) in the… CONTINUE READING
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