LFA-1–mediated Adhesion Is Regulated by Cytoskeletal Restraint and by a Ca2+-dependent Protease, Calpain

@article{Stewart1998LFA1mediatedAI,
  title={LFA-1–mediated Adhesion Is Regulated by Cytoskeletal Restraint and by a Ca2+-dependent Protease, Calpain},
  author={M. Stewart and A. McDowall and N. Hogg},
  journal={The Journal of Cell Biology},
  year={1998},
  volume={140},
  pages={699 - 707}
}
The activity of integrins on leukocytes is kept under tight control to avoid inappropriate adhesion while these cells are circulating in blood or migrating through tissues. Using lymphocyte function-associated antigen-1 (LFA-1) on T cells as a model, we have investigated adhesion to ligand intercellular adhesion molecule-1 induced by the Ca2+ mobilizers, ionomycin, 2,5-di-t-butylhydroquinone, and thapsigargin, and the well studied stimulators such as phorbol ester and cross-linking of the… Expand
SDF-1α (CXCL12) regulation of lateral mobility contributes to activation of LFA-1 adhesion.
TLDR
It is found that SDF-1α releases L FA-1 from cytoskeletal constraints as effectively as does cytochalasin D, and the resultant increased diffusion is correlated with a robust increase in LFA-1-mediated adhesion under physiological shear stress. Expand
Cytoskeletal regulation couples LFA-1 conformational changes to receptor lateral mobility and clustering.
TLDR
Measurements show that different receptor conformations have distinct diffusion profiles and that these profiles vary according to the activation state of the cell, suggesting that current models of L FA-1 regulation are incomplete and that LFA-1 confinement by cytoskeletal attachment regulates cell adhesion both negatively and positively. Expand
Contributions of molecular binding events and cellular compliance to the modulation of leukocyte adhesion
TLDR
The mechanisms by which phorbol myristate acetate (PMA) promotes LFA-1-dependent cell adhesion are investigated and it is estimated that changes in the elasticity of the cell gave rise to a more than 10-fold increase in cellAdhesion. Expand
Integrin Leukocyte Function-associated Antigen-1-mediated Cell Binding Can Be Activated by Clustering of Membrane Rafts*
TLDR
It is reported here that LFA-1-mediated binding of mouse thymocytes or activated T lymphocytes to intercellular adhesion molecule 1 can be rapidly induced by clustering of membrane rafts using antibodies to the glycosylphophatidylinositol-anchored molecule CD24 or cholera toxin (CTx). Expand
Dynamic Regulation of LFA-1 Activation and Neutrophil Arrest on Intercellular Adhesion Molecule 1 (ICAM-1) in Shear Flow*
TLDR
The data reveal that the number of chemotactic receptors bound and the topography and lifetime of high affinity LFA-1 tightly regulate the efficiency of neutrophil capture on ICAM-1. Expand
Nonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration
TLDR
It is shown that nonmuscle myosin heavy chain IIA (MyH9) is recruited to L FA-1 at the uropod of migrating T lymphocytes, and inhibition of the association of MyH9 with LFA-1 results in extreme Uropod elongation, defective tail detachment, and decreased lymphocyte migration on ICAM-1, without affecting LFA -1 activation by chemokine CXCL-12. Expand
LFA‐1 integrin and the microtubular cytoskeleton are involved in the Ca2+‐mediated regulation of the activity of the tyrosine kinase PYK2 in T cells
TLDR
It is shown that Ca2+ mobilizing agents A23187, thapsigargin, and ionomycin induce an increase in adhesion to the LFA‐1 ligand intercellular adhesion molecule‐1 (ICAM‐1) and activation and redistribution of the proline‐rich tyrosine kinase‐2 (PYK2) to the microtubule‐organizing center (MTOC) in T‐lymphoblasts. Expand
Ionomycin-induced activation of LFA-1 is independent of calpain-mediated cleavage of talin
Integrins are heterodimeric surface receptors that play a critical role in many cellular processes due to their ability to mediate the interactions between cells as well as between cells and theExpand
Signaling Through Integrin LFA-1 Leads to Filamentous Actin Polymerization and Remodeling, Resulting in Enhanced T Cell Adhesion1
TLDR
The findings demonstrate that LFA-1 acts not only as an adhesion receptor but also as a signaling receptor by actively initiating the F-actin reorganization that is essential for many T cell-dependent processes. Expand
Comparative studies of LFA-1/ICAM-1 interaction by micropipette and flow chamber techniques.
TLDR
Experimental results show that PHA stimulation of lymphocytes resulted in significant increases in the adhesion probability (Pa) and in number of firmly adhered lymphocytes to HUVECs, but TMP treatment could significantly inhibit such increases. Expand
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Ligand intercellular adhesion molecule 1 has a necessary role in activation of integrin lymphocyte function-associated molecule 1.
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Activation of LFA-1 is in part achieved by an induced fit imposed from without by interaction with ligand, suggesting that, in addition to the inside-out signaling, a previously unrecognized requirement for full activation of the leukocyte integrin LFA -1 is the initial interaction with its ligand ICAM-1. Expand
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