LEAP: L1 Element Amplification Protocol.


Long INterspersed Element-1 (LINE-1 or L1) retrotransposons encode two proteins (ORF1p and ORF2p) that are required for retrotransposition. The L1 element amplification protocol (LEAP) assays the ability of L1 ORF2p to reverse transcribe L1 RNA in vitro. Ultracentrifugation or immunoprecipitation is used to isolate L1 ribonucleoprotein particle (RNP… (More)
DOI: 10.1007/978-1-4939-3372-3_21


Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.